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IL-1β may contribute to NGF-induced alterations in pulmonary hypertension - 17/02/23

Doi : 10.1016/j.rmr.2022.11.036 
C. Bouchet 1, , G. Cardouat 1, P. Fernandes 1, A. Marandeau 1, P. Robillard 1, F. Delcambre 2, R. Marthan 1, 2, P. Berger 1, 2, C. Guibert 1, V. Freund-Michel 1
1 InsermU1045, centre de recherche cardiothoracique de Bordeaux, université Bordeaux, Bordeaux, France 
2 CHU de Bordeaux, France 

Corresponding author.

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Résumé

Introduction

Pulmonary hypertension (PH) is a severe disease leading to right heart failure and death. We have previously shown that blockade of the nerve growth factor NGF may be of therapeutic interest in PH, since NGF contributes to various pathophysiological aspects of this disease [1]. Interleukin-1β (IL-1β) is a pro-inflammatory cytokine whose levels are increased in PH [2]. Our previous experiments have shown that NGF can enhance IL-1β secretion from both pulmonary arterial structural cells and inflammatory cells, i.e. monocytes. In the present study, we have investigated whether IL-1β may play a role in NGF-dependent pulmonary arterial (PA) alterations in PH.

Methods

In vitro, control human PA smooth muscle (hPASMC) or endothelial cells (hPAEC) were treated with IL-1β (10 or 100ng/mL, 24h). Cell proliferation (counting, Ki67 staining), migration (transwell assay), apoptosis (caspase 3,7 activities) and interleukin-6 (IL-6) secretion (ELISA) were then assessed. In hPAEC, expression of endothelial nitric oxide synthase (eNOS) and intercellular adhesion molecule-1 (ICAM-1) were also assessed by Western Blotting. Signalling pathways activated by IL-1β to induce IL-6 secretion were investigated by using PDTC (pyrrolidine dithiocarbamate, an NF-κB inhibitor, 50μM) or SP60025 (an inhibitor of Activator protein 1 [AP-1], 10μM). In vivo, PH was induced in rats by monocrotaline (MCT, 60mg/kg, intraperitoneal injection), and IL-1β plasmatic and pulmonary levels were then determined. Expression of inflammasome components (NLRP3/pro- and active caspase-1/pro-IL-1β) and of CD11b (marker of monocytes/macrophages infiltration) was also assessed in lung and PA homogenates by Western Blotting.

Results

In vitro, IL-1β increased hPASMC and hPAEC proliferation and migration, as well as IL-6 secretion through activation of both NF-κB and AP-1. IL-1β also increased hPASMC resistance to apoptosis, and increased ICAM-1 expression whereas decreased eNOS expression in hPAEC. In vivo, IL-1β plasmatic and pulmonary levels as well as expression of inflammasome components and of CD11b were all increased in PH rats compared to controls.

Conclusion

In conclusion, our results suggest that IL-1β-induced stimulation of both hPASMC and hPAEC may play a role in PA remodelling, inflammation and altered endothelial function. Since NGF participates in IL-1β increased levels in PH, IL-1β may therefore contribute to NGF-dependent PA alterations in this disease.

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© 2022  Publié par Elsevier Masson SAS.
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Vol 40 - N° 2

P. 126 - février 2023 Retour au numéro
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